Pseudomonas putidaKT2440 markerless gene deletion using a combination of λ Red recombineering and Cre/loxPsite-specific recombination
نویسندگان
چکیده
منابع مشابه
λ-Red-Recombineering Live Attenuated ΔipaD Shigella dysenteriae from Iranian Isolates as A Candidate of Vaccine
Shigella species (spp.) are gram-negative bacteria that are responsible for shigellosis. Although it can be controlled via antibiotics, increasing number of antibiotic resistant isolates of Shigella have been reported. Therefore, other strategies such as production of specific vaccine against this organism could be a suitable therapeutic approach. Attenuated live vaccines produced by gene delet...
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The λ-Red recombination system is a popular method for gene editing. However, its applications are limited due to restricted electroporation of DNA fragments. Here, we present an electroporation-free λ-Red recombination method in which target DNA fragments are excised by I-CreI endonuclease in vivo from the landing pad plasmid. Subsequently, the I-SceI endonuclease-cutting chromosome and DNA do...
متن کاملλ-red-recombineering live attenuated δipad shigella dysenteriae from iranian isolates as a candidate of vaccine
shigella species (spp.) are gram-negative bacteria that are responsible for shigellosis. although it can be controlled via antibiotics, increasing number of antibiotic resistant isolates of shigella have been reported. therefore, other strategies such as production of specific vaccine against this organism could be a suitable therapeutic approach. attenuated live vaccines produced by gene delet...
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In this report, we describe the identification of functions that promote genomic recombination of linear DNA introduced into Pseudomonas cells by electroporation. The genes encoding these functions were identified in Pseudomonas syringae pv. syringae B728a based on similarity to the lambda Red Exo/Beta and RecET proteins encoded by the lambda and Rac bacteriophages of Escherichia coli. The abil...
متن کاملRecombineering: genetic engineering in bacteria using homologous recombination.
The bacterial chromosome and plasmids can be engineered in vivo by homologous recombination using PCR products and synthetic oligonucleotides as substrates. This is possible because bacteriophage-encoded recombination functions efficiently to recombine sequences with homologies as short as 35 to 40 bases. This recombineering allows DNA sequences to be inserted or deleted without regard to locat...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2016
ISSN: 1574-6968
DOI: 10.1093/femsle/fnw014